Optimization and validation of ultrasensitive GC–MS/MS method to measure oxidatively induced DNA damage products and role of antioxidants in oxidation mechanism

Oxidation of DNA due to exposure reactive oxygen species (ROS) is a major source damage. ROS induced damage plays an important role in some diseases such as various cancers, aging and neurodegenerative diseases. The detection oxidation products assessing the mutagenicity potential specific exposure. GC–MS/MS method was developed for ultrasensitive determination individual products. validation results revealed that proposed reliable sensitive. Multiple response surface methodology (MRSM) used optimize derivatization conditions oxidatively base before analysis. optimum were determined 40 min time, 120 °C temperature 1.4 BSTFA/pyridine ratio under nitrogen atmosphere. effects thymol, carvacrol thymoquinone antioxidants investigated on oxidative performed after adding with different concentrations stress. Eighteen analyzed simultaneously using GC–MS/MS. This study showed significant decrease amount when present medium.